cellassign

cellassign automatically assigns single-cell RNA-seq data to known cell types across thousands of cells accounting for patient and batch specific effects. Information about a priori known markers cell types is provided as input to the model in the form of a (binary) marker gene by cell-type matrix. cellassign then probabilistically assigns each cell to a cell type, removing subjective biases from typical unsupervised clustering workflows.

Getting started

Installation

Installing from GitHub

cellassign is built using Google’s Tensorflow, and as such requires installation of the R package tensorflow:

install.packages("tensorflow")
tensorflow::install_tensorflow(extra_packages='tensorflow-probability')

Please ensure this installs version 2 of tensorflow. You can check this by calling

tensorflow::tf_config()

TensorFlow v2.0.0 (/usr/local/lib/python3.7/site-packages/tensorflow)

cellassign can then be installed from github:

install.packages("devtools") # If not already installed
devtools::install_github("Irrationone/cellassign")

Installing from conda

With conda, install the current release version of cellassign as follows:

conda install -c conda-forge -c bioconda r-cellassign

Vignettes

• Assigning single-cells to known cell types with CellAssign

• Constructing marker genes from purified bulk/scRNA-seq data

Basic usage

cellassign requires the following inputs:

• exprs_obj: Cell-by-gene matrix of raw counts (or SingleCellExperiment with counts assay)
• marker_gene_info: Binary gene-by-celltype marker gene matrix or list relating cell types to marker genes
• s: Size factors
• X: Design matrix for any patient/batch specific effects

The model can be run as follows:

cas <- cellassign(exprs_obj = gene_expression_data,
                  marker_gene_info = marker_gene_info,
                  s = s,
                  X = X)

An example set of markers for the human tumour microenvironment can be loaded by calling

data(example_TME_markers)

Please see the package vignette for details and caveats.

Paper

Probabilistic cell-type assignment of single-cell RNA-seq for tumor microenvironment profiling, Nature Methods 2019

Authors

Allen W Zhang, University of British Columbia

Kieran R Campbell, University of British Columbia